Organoid & primary cell culture
Experience with intestinal crypt isolation and organoid culture, including Matrigel-based plating, preparation of WENR/ENR culture conditions, growth-factor supplementation, and clonogenic organoid assays. Applied isolated intestinal stem-cell and Paneth-cell populations to questions of epithelial regeneration and cellular plasticity.
Fluorescence-activated cell sorting
Experience isolating intestinal epithelial populations using Sox9EGFP and cell identity, with sorted populations used in downstream clonogenic culture experiments examining stem-cell behavior and Paneth-cell dedifferentiation.
Immunofluorescence & tissue imaging
Design and execution of intestinal-tissue immunofluorescence experiments, including cryosectioning, antigen retrieval, antibody staining, fluorescence microscopy, and quantitative image analysis. Worked with lineage markers including CHGA, MUC2, DCAMKL1, and lysozyme.
HCR RNA-FISH
Experience using HCR fluorescence in situ hybridization to visualize RNA expression in intestinal epithelial tissue and cell models, supporting spatial characterization of epithelial cell states.
Mouse intestinal tissue processing
Experience with intestinal dissection, tissue preparation, cryosectioning, and histological analysis in genetically engineered mouse models used to study intestinal stem-cell injury and regeneration.
Quantitative microscopy & analysis
Quantified fluorescence-based phenotypes across intestinal and neural tissue, including cell abundance, spatial distribution, marker expression, amyloid-β accumulation, and microglial responses.
Experimental design & data analysis
Experience organizing replicate-level datasets, performing statistical comparisons, interpreting biological phenotypes, and connecting quantitative results to mechanistic questions.
Genetic evidence curation
Literature-based curation of GRIN1, GRIN2A, and GRIN2B variants for gene–disease validity assessment and clinical interpretation through ClinGen annotation research.